LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒 LBIS® OVA-IgE Mouse

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LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒
LBIS® OVA-IgE Mouse

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LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒                              LBIS® OVA-IgE Mouse小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒

 


  IgE(Immunoglobulin E,免疫球蛋白E)是第五个被发现的免疫球蛋白,由5个结构域(VH、CHε 1~4)构成的两个Hε链和两个L链中组成的IgE,其分子量约190000,电泳实验中向γ1域移动。IgE的代谢半衰期约3天,正常人血清中的IgE浓度非常低,约300 ng/mL。但在寄生虫感染和枯草热时浓度会升高。与过敏性相关的IgE被称作反应素。因接触过敏原导致反应素含量升高,反应素会在Fc域与存在于皮肤、呼吸道、消化脏器中嗜碱性粒细胞和肥大细胞的FcεR1受体结合,引起细胞过敏。在结合过敏原后细胞发生脱粒现象,组胺,五羟色胺,蛋白酶,肝素,趋化因子,前列腺素,白三烯等被投放,经过支气管收缩和黏膜水肿,分泌亢进,从而诱发支气管哮喘,部分荨麻疹,过敏性鼻炎,过敏性反应等I型过敏性反应。

  本试剂盒是以OVA(卵清蛋白)作为免疫的抗原,在一个简化的反应系统中通过测定鼠抗OVA- IgE抗体值来进行小鼠免疫系统检测的试剂盒。

◆特点

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒                              LBIS® OVA-IgE Mouse


● 测定时间短(总反应时间:1小时50分钟)。

● 微量样本即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

● 操作简便,无需进行特殊前处理。

 

试剂盒组成


组成品

状态

包装

OVA包被96孔板(干燥板)

清洗后使用

96 wells(8×12)/1个

标准溶液(Anti OVA-IgE:1,200 U/mL)(单抗)

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗小鼠IgE抗体(单抗)

稀释后使用

200 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

200 μL/1瓶

显色液(TMB)

直接使用

12 mL/1瓶

终止液(1M H2SO4)※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

 


◆样本信息


● 小鼠血清·血浆

● 10 μL/well(稀释样本)

※ 样本需要用试剂盒附带的缓冲液调至标准曲线范围内。

※ 样本必须稀释10倍以上。

 


测定范围


1.88~120 U/mL(标准曲线范围)

(本试剂盒中1 U/mL定义为抗原结合常数(Ka)为2.0×108 M-1的抗体1.3 ng/mL)

 


实验数据


精度测试(组内变异)


样本

A

B

1

70.7

19.1

2

71.0

18.7

3

77.1

19.6

4

74.3

19.7

5

72.9

18.9

Mean

73.2

19.2

SD

2.6

0.42

CV(%)

3.6

2.2

单位:U/mL



重复性测试(组间变异)


测定日/样本

C

D

E

0天

60.0

15.0

3.75

1天

59.1

15.0

3.75

2天

58.1

14.7

3.65

3天

63.6

16.0

3.48

Mean

60.2

15.2

3.66

SD

2.4

0.57

0.13

CV(%)

4.0

3.7

3.4

单位:U/mL



加标回收测试


样本F


添加量

实测值

回收量

回收率(%)

0.00

6.93

5.35

12.2

5.27

98.5

10.7

17.8

10.9

102

17.1

25.1

18.2

106

单位:U/mL,n=3


样本G


添加量

实测值

回收量

回收率(%)

0.00

40.5

30.8

70.4

29.5

95.8

35.9

77.1

36.6

102

53.9

94.9

54.4

101

单位:U/mL,n=3



稀释直线性测试


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值R2=0.9987~0.9999

参考文献



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Pinocembrin attenuates allergic airway inflammation via inhibition of NF-κB pathway in mice. Gu X, Zhang Q, Du Q, Shen H, Zhu Z. Int Immunopharmacol. 2017 Oct 18;53:90-95.


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A dichloromethane fraction of Triticum aestivum sprouts reduces allergic immune response through inhibiting Th2 differentiation in ovalbumin‑immunized mice. Ki HH, Hwang SW, Lee JH, Kim YH, Kim DK, Lee YM. Mol Med Rep. 2017 Sep;16(3):3535-3541.


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Urban PM2.5 exacerbates allergic inflammation in the murine lung via a TLR2/TLR4/MyD88-signaling pathway. He M, Ichinose T, Yoshida Y, Arashidani K, Yoshida S, Takano H, Sun G, Shibamoto T. Sci Rep. 2017 Sep 8;7(1):11027.


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Activation of group 2 innate lymphoid cells exacerbates and confers corticosteroid resistance to mouse nasal type 2 inflammation. Morikawa T, Fukuoka A, Matsushita K, Yasuda K, Iwasaki N, Akasaki S, Fujieda S, Yoshimoto T. Int Immunol. 2017 May 1;29(5):221-233.


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Exposure to bisphenol A enhanced lung eosinophilia in adult male mice. He M, Ichinose T, Yoshida S, Takano H, Nishikawa M, Shibamoto T, Sun G. Allergy Asthma Clin Immunol. 2016 Apr 14;12:16.


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Differences in allergic inflammatory responses between urban PM2.5 and fine particle derived from desert-dust in murine lungs. He M, Ichinose T, Kobayashi M, Arashidani K, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T. Toxicol Appl Pharmacol. 2016 Apr 15;297:41-55.


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Desert dust induces TLR signaling to trigger Th2-dominant lung allergic inflammation via a MyD88-dependent signaling pathway. He M, Ichinose T, Song Y, Yoshida Y, Bekki K, Arashidani K, Yoshida S, Nishikawa M, Takano H, Shibamoto T, Sun G. Toxicol Appl Pharmacol. 2016 Apr 1;296:61-72.


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19.

Pharyngeal aspiration of metal oxide nanoparticles showed potential of allergy aggravation effect to inhaled ovalbumin. Horie M, Stowe M, Tabei M, Kuroda E. Inhal Toxicol. Vol.27(3), p181-90, Feb 2015.


20.

Oxidized dietary oils enhance immediate- and/or delayed-type allergic reactions in BALB/c mice. Ogino H, Sakazaki F, Okuno T, Arakawa T, Ueno H. Allergol Int. Vol.64(1), p66-72, Jan 2015.


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22.

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A bacterial extract of OM-85 Broncho-Vaxom prevents allergic rhinitis in mice. Han L, Zheng CP, Sun YQ, Xu G, Wen W, Fu QL. American Journal of Rhinology & Allergy, Vol.28(2), p110-116, Mar-Apr 2014.


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27.

Lung inflammation by fungus, Bjerkandera adusta isolated from Asian sand dust (ASD) aerosol and enhancement of ovalbumin-induced lung eosinophilia by ASD and the fungus in mice. Liu B, Ichinose T, He M, Kobayashi F, Maki T, Yoshida S, Yoshida Y, Arashidani K, Takano H, Nishikawa M, Sun G, Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.10(1), Feb 2014.


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29.

Induction of immune tolerance and reduction of aggravated lung eosinophilia by co-exposure to Asian sand dust and ovalbumin for 14 weeks in mice. He M., Ichinose T., Yoshida S., Takano H., Nishikawa M., Sun G. and Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.9(19), 2013.


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38.

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Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T Cells in Th2-Type Airway Inflammation. Saito, K., Torii, M., Ning Ma, Tsuchiya, T., Wang, L., Hori, T., Nagakubo, D., Nitta, N.,Kanegasaki, S., Hieshima, K., Yoshie, O., Gabazza, E.C., Katayama, N., Shiku, H.,Kuribayashi, K. and Kato, T. The Journal of Immunology, 181:6889-6897, 2008


53.

Effects of Asian Sand Dust, Arizona Sand Dust, Amorphous Silica and Aluminum Oxide on Allergic Inflammation in the Murine Lung. Ichinose, T., Yoshida, S., Sadakane, K., Takano, H., Yanagizawa, R., Inoue, K., Nishikawa, M.,Mori, I., Kawazato, H., Yasuda, A. and Shibamoto, T. Inhalation Toxicology, Volume 20, Issue 7, 685-694, 2008


54.

The Effects of Microbial Materials Adhered to Asian Sand Dust on Allergic Lung Inflammation. Ichinose,T., Yoshida,S., Hiyoshi,K., Sadakane,K., Takano,H., Nishikawa,M., Mori,I., Yanagisawa,R., Kawazato,H., Yasuda,A., and Shibamoto,T. Arch Environ Contam Toxicol 55:348-357,2008


55.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T cell in Th2-Type Airway Inflammation. Saito,K., Torii,M., Ma,N., Tsuchiya,T., Wang,L.,Hori,T., Nagakubo,D., Nitta,N., Kanegasaki,S., Hieshima,K., Yoshie,O., Gabazza,E,C., Katayama,N., Shiku,H., Kuribayashi,K., and Kato,T. The Journal of Immunology 181:6889-6897,2008



产品列表

产品编号 产品名称 产品规格 产品等级 备注
639-07651 (AKRIE-030)小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒
 LBIS® OVA-IgE Mouse
96 tests

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LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒 LBIS® OVA-IgG1 Mouse

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LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒
LBIS® OVA-IgG1 Mouse

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒                              LBIS® OVA-IgG1 MouseLBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒

 


  IgG是血清中免疫球蛋白中含量最高的二次免疫应答主要抗体。占据三分之二IgG的是具有γ1的H链,分子量146 kDa的IgG1。以OVA(卵清蛋白)作为特殊化抗原,通过简化测定抗OVA- IgG1抗体值来阐明小鼠免疫系统作用机理。

  小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒是仅特异性测定Anit-OVA-IgG1的试剂盒。

 

◆特点

LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒                              LBIS® OVA-IgG1 Mouse


● 测定时间短(总反应时间:1小时50分钟)

● 微量样本即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

● 操作简便,无特殊前处理。

 



试剂盒组成


组成品

状态

包装

OVA包被96孔板(干燥板)

清洗后使用

96 wells(8×12)/1个

标准溶液(Anti OVA- IgG1:1,200U/mL)(单抗)

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗小鼠IgG1抗体(单抗)

稀释后使用

200 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

200 μL/1瓶

显色液(TMB)

直接使用

12 mL/1瓶

终止液(1M H2SO4

※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

 


样本信息

● 小鼠血清·血浆

● 10 μL/well(稀释样本)

※ 样本必须用附带的缓冲液稀释100倍以上。

 


◆测定范围


1.88~120 mU/mL(标准曲线范围)

(本试剂盒中1 U/mL定义为抗原结合常数(Ka)为6.9×107 M-1的抗体160ng/mL)

 


实验数据


精度测试(组内变异)


样本

A

B

1

105

18.2

2

100

16.9

3

96.2

16.7

4

100

17.4

5

106

17.4

Mean

101

17.3

SD

4.0

0.59

CV(%)

4.0

3.4

单位:mU/mL



重复性测试(组间变异)


测定日/样本

C

D

E

0天

60.1

15.0

3.75

1天

58.5

14.7

3.70

2天

59.9

15.3

3.83

3天

61.4

15.7

3.82

Mean

60.0

15.2

3.77

SD

1.2

0.44

0.06

CV(%)

2.0

2.9

1.6

单位:mU/mL



加标回收测试


样本F


添加量

实测值

回收量

回收率(%)

0.00

8.86

7.29

15.8

6.94

95.2

11.0

19.5

10.6

96.4

14.6

23.0

14.1

96.6

单位:mU/mL,n=3


样本G


添加量

实测值

回收量

回收率(%)

0.00

51.5

26.9

76.8

25.3

97.1

31.4

80.6

29.1

92.5

47.1

100

48.5

103

单位:mU/mL,n=3



稀释直线性测试


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值R2=0.9994~0.9998

参考文献



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Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T Cells in Th2-Type Airway Inflammation. Saito, K., Torii, M., Ning Ma, Tsuchiya, T., Wang, L., Hori, T., Nagakubo, D., Nitta, N.,Kanegasaki, S., Hieshima, K., Yoshie, O., Gabazza, E.C., Katayama, N., Shiku, H.,Kuribayashi, K. and Kato, T. The Journal of Immunology, 181:6889-6897, 2008


53.

Effects of Asian Sand Dust, Arizona Sand Dust, Amorphous Silica and Aluminum Oxide on Allergic Inflammation in the Murine Lung. Ichinose, T., Yoshida, S., Sadakane, K., Takano, H., Yanagizawa, R., Inoue, K., Nishikawa, M.,Mori, I., Kawazato, H., Yasuda, A. and Shibamoto, T. Inhalation Toxicology, Volume 20, Issue 7, 685-694, 2008


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The Effects of Microbial Materials Adhered to Asian Sand Dust on Allergic Lung Inflammation. Ichinose,T., Yoshida,S., Hiyoshi,K., Sadakane,K., Takano,H., Nishikawa,M., Mori,I., Yanagisawa,R., Kawazato,H., Yasuda,A., and Shibamoto,T. Arch Environ Contam Toxicol 55:348-357,2008


55.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T cell in Th2-Type Airway Inflammation. Saito,K., Torii,M., Ma,N., Tsuchiya,T., Wang,L.,Hori,T., Nagakubo,D., Nitta,N., Kanegasaki,S., Hieshima,K., Yoshie,O., Gabazza,E,C., Katayama,N., Shiku,H., Kuribayashi,K., and Kato,T. The Journal of Immunology 181:6889-6897,2008



产品列表

产品编号 产品名称 产品规格 产品等级 备注
630-07669 (AKRIE-040)小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1) ELISA试剂盒
LBIS® OVA-IgG1 Mouse
96 tests

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